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Accurate 96, Real-Time PCR System, DLAB

• Up to 6 fluorescence detection channels allowing multiplex PCR.
• Effectively reduce multi-color crosstalk and edge effect, no ROX correction required.
• New optical scanning detection system
• Innovative scanning method and time-resolved signal separation technology
• Unique edge temperature compensation technology
• User-friendly software

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Description

Temperature Control System

Sample Capacity 0.1ml PCR tubes×96, 8×12 PCR
plate or 96 well plate ×1
Reaction volume 10-50 μl
Thermal cycle technology Peltier
Max. Heating/Cooling rate 6.0° C/s
Heating temperature range 4 – 100 °C
Temperature accuracy ± 0.2°C
Temperature uniformity ±0.2℃ @60℃ , ±0.2℃ @95℃
Temperature gradient setting range 30–100°C
Temperature gradient difference setting range 1 – 36°C

Detection System

Excitation light source 4/6 monochrome high efficiency LEDs
Detection device PMT
Detection mode Time-resolved signal separating technology
Excitation/detection wavelength range 455-650nm/510-715nm
Fluorescent channels 4/6 channels
Supported dye FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/ Texas Red, Mustang Purple, Cy5/LIZ
Sensitivity Single copy gene
Resolution 1.33 folds copy number difference can be distinguished in single-plex qPCR
Dynamic range 10 orders of magnitude copies